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Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:a hundred dilution. Incubate the membrane for 20 min and then wash with DMF to remove uncoupled amino acids. Note that the facet chain has three methylene teams, so that even though the terminal amino group might be charged beneath physiological circumstances, the side chain does have vital hydrophobic character.